fragmentation buffer Search Results


proprietary fragmentation buffer  (Illumina Inc)
90
Illumina Inc proprietary fragmentation buffer
Proprietary Fragmentation Buffer, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/proprietary fragmentation buffer/product/Illumina Inc
Average 90 stars, based on 1 article reviews
proprietary fragmentation buffer - by Bioz Stars, 2026-04
90/100 stars
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fragmentation buffer  (10X Genomics)
90
10X Genomics fragmentation buffer
Overview of the protocol and setup. A) Schematic of the entire workflow; the protocol is divided into two parts, one manual and one automated. The manual part starts with tissue sections already sectioned and placed onto the Visium Gene Expression array. Day 1 (manual part) is performed on the array until cDNA denaturation, after which the material is released. The manual part ends with a quality control to ensure good libraries before continuing with the library construction. Day 2 (automated part) consists of library construction which begins with <t>fragmentation</t> and also ends with a quality control to determine library sizes. B) Layout of the working deck of the Agilent Bravo Liquid Handling Platform. Prior to starting the automated part, the working deck is loaded with a full tip box on position 2, an empty Nunc deep well 1.3 ml plate on position 3, a 2.2 ml deep well storage plate containing room temperature reagents on position 5, an empty 96-well PCR plate on position 6, an empty tip box on position 8 and a 96-well PCR plate containing the samples and reagents on position 9. C) Schematic of the division of samples to technical replicates for the comparison experiments. Well A1 of the Visium Spatial Gene Expression array is used as an example.
Fragmentation Buffer, supplied by 10X Genomics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fragmentation buffer/product/10X Genomics
Average 90 stars, based on 1 article reviews
fragmentation buffer - by Bioz Stars, 2026-04
90/100 stars
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fragmentation buffer  (Illumina Inc)
90
Illumina Inc fragmentation buffer
Overview of the protocol and setup. A) Schematic of the entire workflow; the protocol is divided into two parts, one manual and one automated. The manual part starts with tissue sections already sectioned and placed onto the Visium Gene Expression array. Day 1 (manual part) is performed on the array until cDNA denaturation, after which the material is released. The manual part ends with a quality control to ensure good libraries before continuing with the library construction. Day 2 (automated part) consists of library construction which begins with <t>fragmentation</t> and also ends with a quality control to determine library sizes. B) Layout of the working deck of the Agilent Bravo Liquid Handling Platform. Prior to starting the automated part, the working deck is loaded with a full tip box on position 2, an empty Nunc deep well 1.3 ml plate on position 3, a 2.2 ml deep well storage plate containing room temperature reagents on position 5, an empty 96-well PCR plate on position 6, an empty tip box on position 8 and a 96-well PCR plate containing the samples and reagents on position 9. C) Schematic of the division of samples to technical replicates for the comparison experiments. Well A1 of the Visium Spatial Gene Expression array is used as an example.
Fragmentation Buffer, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fragmentation buffer/product/Illumina Inc
Average 90 stars, based on 1 article reviews
fragmentation buffer - by Bioz Stars, 2026-04
90/100 stars
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5× fragmentation buffer  (Illumina Inc)
90
Illumina Inc 5× fragmentation buffer
Overview of the protocol and setup. A) Schematic of the entire workflow; the protocol is divided into two parts, one manual and one automated. The manual part starts with tissue sections already sectioned and placed onto the Visium Gene Expression array. Day 1 (manual part) is performed on the array until cDNA denaturation, after which the material is released. The manual part ends with a quality control to ensure good libraries before continuing with the library construction. Day 2 (automated part) consists of library construction which begins with <t>fragmentation</t> and also ends with a quality control to determine library sizes. B) Layout of the working deck of the Agilent Bravo Liquid Handling Platform. Prior to starting the automated part, the working deck is loaded with a full tip box on position 2, an empty Nunc deep well 1.3 ml plate on position 3, a 2.2 ml deep well storage plate containing room temperature reagents on position 5, an empty 96-well PCR plate on position 6, an empty tip box on position 8 and a 96-well PCR plate containing the samples and reagents on position 9. C) Schematic of the division of samples to technical replicates for the comparison experiments. Well A1 of the Visium Spatial Gene Expression array is used as an example.
5× Fragmentation Buffer, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/5× fragmentation buffer/product/Illumina Inc
Average 90 stars, based on 1 article reviews
5× fragmentation buffer - by Bioz Stars, 2026-04
90/100 stars
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rna fragmentation buffer  (Ribobio co)
90
Ribobio co rna fragmentation buffer
Overview of the protocol and setup. A) Schematic of the entire workflow; the protocol is divided into two parts, one manual and one automated. The manual part starts with tissue sections already sectioned and placed onto the Visium Gene Expression array. Day 1 (manual part) is performed on the array until cDNA denaturation, after which the material is released. The manual part ends with a quality control to ensure good libraries before continuing with the library construction. Day 2 (automated part) consists of library construction which begins with <t>fragmentation</t> and also ends with a quality control to determine library sizes. B) Layout of the working deck of the Agilent Bravo Liquid Handling Platform. Prior to starting the automated part, the working deck is loaded with a full tip box on position 2, an empty Nunc deep well 1.3 ml plate on position 3, a 2.2 ml deep well storage plate containing room temperature reagents on position 5, an empty 96-well PCR plate on position 6, an empty tip box on position 8 and a 96-well PCR plate containing the samples and reagents on position 9. C) Schematic of the division of samples to technical replicates for the comparison experiments. Well A1 of the Visium Spatial Gene Expression array is used as an example.
Rna Fragmentation Buffer, supplied by Ribobio co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rna fragmentation buffer/product/Ribobio co
Average 90 stars, based on 1 article reviews
rna fragmentation buffer - by Bioz Stars, 2026-04
90/100 stars
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fragmentation buffer/blocking buffer  (Arraystar inc)
90
Arraystar inc fragmentation buffer/blocking buffer
Overview of the protocol and setup. A) Schematic of the entire workflow; the protocol is divided into two parts, one manual and one automated. The manual part starts with tissue sections already sectioned and placed onto the Visium Gene Expression array. Day 1 (manual part) is performed on the array until cDNA denaturation, after which the material is released. The manual part ends with a quality control to ensure good libraries before continuing with the library construction. Day 2 (automated part) consists of library construction which begins with <t>fragmentation</t> and also ends with a quality control to determine library sizes. B) Layout of the working deck of the Agilent Bravo Liquid Handling Platform. Prior to starting the automated part, the working deck is loaded with a full tip box on position 2, an empty Nunc deep well 1.3 ml plate on position 3, a 2.2 ml deep well storage plate containing room temperature reagents on position 5, an empty 96-well PCR plate on position 6, an empty tip box on position 8 and a 96-well PCR plate containing the samples and reagents on position 9. C) Schematic of the division of samples to technical replicates for the comparison experiments. Well A1 of the Visium Spatial Gene Expression array is used as an example.
Fragmentation Buffer/Blocking Buffer, supplied by Arraystar inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fragmentation buffer/blocking buffer/product/Arraystar inc
Average 90 stars, based on 1 article reviews
fragmentation buffer/blocking buffer - by Bioz Stars, 2026-04
90/100 stars
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hybridisation mixture containing hybridisation buffer, fragmented crna (0.05 µg/µl) and herring sperm dna (0.1 mg/ml  (Promega)
90
Promega hybridisation mixture containing hybridisation buffer, fragmented crna (0.05 µg/µl) and herring sperm dna (0.1 mg/ml
Overview of the protocol and setup. A) Schematic of the entire workflow; the protocol is divided into two parts, one manual and one automated. The manual part starts with tissue sections already sectioned and placed onto the Visium Gene Expression array. Day 1 (manual part) is performed on the array until cDNA denaturation, after which the material is released. The manual part ends with a quality control to ensure good libraries before continuing with the library construction. Day 2 (automated part) consists of library construction which begins with <t>fragmentation</t> and also ends with a quality control to determine library sizes. B) Layout of the working deck of the Agilent Bravo Liquid Handling Platform. Prior to starting the automated part, the working deck is loaded with a full tip box on position 2, an empty Nunc deep well 1.3 ml plate on position 3, a 2.2 ml deep well storage plate containing room temperature reagents on position 5, an empty 96-well PCR plate on position 6, an empty tip box on position 8 and a 96-well PCR plate containing the samples and reagents on position 9. C) Schematic of the division of samples to technical replicates for the comparison experiments. Well A1 of the Visium Spatial Gene Expression array is used as an example.
Hybridisation Mixture Containing Hybridisation Buffer, Fragmented Crna (0.05 µg/µl) And Herring Sperm Dna (0.1 Mg/Ml, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hybridisation mixture containing hybridisation buffer, fragmented crna (0.05 µg/µl) and herring sperm dna (0.1 mg/ml/product/Promega
Average 90 stars, based on 1 article reviews
hybridisation mixture containing hybridisation buffer, fragmented crna (0.05 µg/µl) and herring sperm dna (0.1 mg/ml - by Bioz Stars, 2026-04
90/100 stars
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short fragment buffer exp-sfb001  (Oxford Nanopore)
90
Oxford Nanopore short fragment buffer exp-sfb001
Overview of the protocol and setup. A) Schematic of the entire workflow; the protocol is divided into two parts, one manual and one automated. The manual part starts with tissue sections already sectioned and placed onto the Visium Gene Expression array. Day 1 (manual part) is performed on the array until cDNA denaturation, after which the material is released. The manual part ends with a quality control to ensure good libraries before continuing with the library construction. Day 2 (automated part) consists of library construction which begins with <t>fragmentation</t> and also ends with a quality control to determine library sizes. B) Layout of the working deck of the Agilent Bravo Liquid Handling Platform. Prior to starting the automated part, the working deck is loaded with a full tip box on position 2, an empty Nunc deep well 1.3 ml plate on position 3, a 2.2 ml deep well storage plate containing room temperature reagents on position 5, an empty 96-well PCR plate on position 6, an empty tip box on position 8 and a 96-well PCR plate containing the samples and reagents on position 9. C) Schematic of the division of samples to technical replicates for the comparison experiments. Well A1 of the Visium Spatial Gene Expression array is used as an example.
Short Fragment Buffer Exp Sfb001, supplied by Oxford Nanopore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/short fragment buffer exp-sfb001/product/Oxford Nanopore
Average 90 stars, based on 1 article reviews
short fragment buffer exp-sfb001 - by Bioz Stars, 2026-04
90/100 stars
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l-fragment buffer lfbd104  (Oxford Nanopore)
90
Oxford Nanopore l-fragment buffer lfbd104
Overview of the protocol and setup. A) Schematic of the entire workflow; the protocol is divided into two parts, one manual and one automated. The manual part starts with tissue sections already sectioned and placed onto the Visium Gene Expression array. Day 1 (manual part) is performed on the array until cDNA denaturation, after which the material is released. The manual part ends with a quality control to ensure good libraries before continuing with the library construction. Day 2 (automated part) consists of library construction which begins with <t>fragmentation</t> and also ends with a quality control to determine library sizes. B) Layout of the working deck of the Agilent Bravo Liquid Handling Platform. Prior to starting the automated part, the working deck is loaded with a full tip box on position 2, an empty Nunc deep well 1.3 ml plate on position 3, a 2.2 ml deep well storage plate containing room temperature reagents on position 5, an empty 96-well PCR plate on position 6, an empty tip box on position 8 and a 96-well PCR plate containing the samples and reagents on position 9. C) Schematic of the division of samples to technical replicates for the comparison experiments. Well A1 of the Visium Spatial Gene Expression array is used as an example.
L Fragment Buffer Lfbd104, supplied by Oxford Nanopore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/l-fragment buffer lfbd104/product/Oxford Nanopore
Average 90 stars, based on 1 article reviews
l-fragment buffer lfbd104 - by Bioz Stars, 2026-04
90/100 stars
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short fragment buffer  (Oxford Nanopore)
90
Oxford Nanopore short fragment buffer
Overview of the protocol and setup. A) Schematic of the entire workflow; the protocol is divided into two parts, one manual and one automated. The manual part starts with tissue sections already sectioned and placed onto the Visium Gene Expression array. Day 1 (manual part) is performed on the array until cDNA denaturation, after which the material is released. The manual part ends with a quality control to ensure good libraries before continuing with the library construction. Day 2 (automated part) consists of library construction which begins with <t>fragmentation</t> and also ends with a quality control to determine library sizes. B) Layout of the working deck of the Agilent Bravo Liquid Handling Platform. Prior to starting the automated part, the working deck is loaded with a full tip box on position 2, an empty Nunc deep well 1.3 ml plate on position 3, a 2.2 ml deep well storage plate containing room temperature reagents on position 5, an empty 96-well PCR plate on position 6, an empty tip box on position 8 and a 96-well PCR plate containing the samples and reagents on position 9. C) Schematic of the division of samples to technical replicates for the comparison experiments. Well A1 of the Visium Spatial Gene Expression array is used as an example.
Short Fragment Buffer, supplied by Oxford Nanopore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/short fragment buffer/product/Oxford Nanopore
Average 90 stars, based on 1 article reviews
short fragment buffer - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
long fragment buffer  (Oxford Nanopore)
90
Oxford Nanopore long fragment buffer
Overview of the protocol and setup. A) Schematic of the entire workflow; the protocol is divided into two parts, one manual and one automated. The manual part starts with tissue sections already sectioned and placed onto the Visium Gene Expression array. Day 1 (manual part) is performed on the array until cDNA denaturation, after which the material is released. The manual part ends with a quality control to ensure good libraries before continuing with the library construction. Day 2 (automated part) consists of library construction which begins with <t>fragmentation</t> and also ends with a quality control to determine library sizes. B) Layout of the working deck of the Agilent Bravo Liquid Handling Platform. Prior to starting the automated part, the working deck is loaded with a full tip box on position 2, an empty Nunc deep well 1.3 ml plate on position 3, a 2.2 ml deep well storage plate containing room temperature reagents on position 5, an empty 96-well PCR plate on position 6, an empty tip box on position 8 and a 96-well PCR plate containing the samples and reagents on position 9. C) Schematic of the division of samples to technical replicates for the comparison experiments. Well A1 of the Visium Spatial Gene Expression array is used as an example.
Long Fragment Buffer, supplied by Oxford Nanopore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/long fragment buffer/product/Oxford Nanopore
Average 90 stars, based on 1 article reviews
long fragment buffer - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
rna fragmentation buffer  (Merck & Co)
90
Merck & Co rna fragmentation buffer
Overview of the protocol and setup. A) Schematic of the entire workflow; the protocol is divided into two parts, one manual and one automated. The manual part starts with tissue sections already sectioned and placed onto the Visium Gene Expression array. Day 1 (manual part) is performed on the array until cDNA denaturation, after which the material is released. The manual part ends with a quality control to ensure good libraries before continuing with the library construction. Day 2 (automated part) consists of library construction which begins with <t>fragmentation</t> and also ends with a quality control to determine library sizes. B) Layout of the working deck of the Agilent Bravo Liquid Handling Platform. Prior to starting the automated part, the working deck is loaded with a full tip box on position 2, an empty Nunc deep well 1.3 ml plate on position 3, a 2.2 ml deep well storage plate containing room temperature reagents on position 5, an empty 96-well PCR plate on position 6, an empty tip box on position 8 and a 96-well PCR plate containing the samples and reagents on position 9. C) Schematic of the division of samples to technical replicates for the comparison experiments. Well A1 of the Visium Spatial Gene Expression array is used as an example.
Rna Fragmentation Buffer, supplied by Merck & Co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rna fragmentation buffer/product/Merck & Co
Average 90 stars, based on 1 article reviews
rna fragmentation buffer - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier

Image Search Results


Overview of the protocol and setup. A) Schematic of the entire workflow; the protocol is divided into two parts, one manual and one automated. The manual part starts with tissue sections already sectioned and placed onto the Visium Gene Expression array. Day 1 (manual part) is performed on the array until cDNA denaturation, after which the material is released. The manual part ends with a quality control to ensure good libraries before continuing with the library construction. Day 2 (automated part) consists of library construction which begins with fragmentation and also ends with a quality control to determine library sizes. B) Layout of the working deck of the Agilent Bravo Liquid Handling Platform. Prior to starting the automated part, the working deck is loaded with a full tip box on position 2, an empty Nunc deep well 1.3 ml plate on position 3, a 2.2 ml deep well storage plate containing room temperature reagents on position 5, an empty 96-well PCR plate on position 6, an empty tip box on position 8 and a 96-well PCR plate containing the samples and reagents on position 9. C) Schematic of the division of samples to technical replicates for the comparison experiments. Well A1 of the Visium Spatial Gene Expression array is used as an example.

Journal: Heliyon

Article Title: Enabling automated and reproducible spatially resolved transcriptomics at scale

doi: 10.1016/j.heliyon.2022.e09651

Figure Lengend Snippet: Overview of the protocol and setup. A) Schematic of the entire workflow; the protocol is divided into two parts, one manual and one automated. The manual part starts with tissue sections already sectioned and placed onto the Visium Gene Expression array. Day 1 (manual part) is performed on the array until cDNA denaturation, after which the material is released. The manual part ends with a quality control to ensure good libraries before continuing with the library construction. Day 2 (automated part) consists of library construction which begins with fragmentation and also ends with a quality control to determine library sizes. B) Layout of the working deck of the Agilent Bravo Liquid Handling Platform. Prior to starting the automated part, the working deck is loaded with a full tip box on position 2, an empty Nunc deep well 1.3 ml plate on position 3, a 2.2 ml deep well storage plate containing room temperature reagents on position 5, an empty 96-well PCR plate on position 6, an empty tip box on position 8 and a 96-well PCR plate containing the samples and reagents on position 9. C) Schematic of the division of samples to technical replicates for the comparison experiments. Well A1 of the Visium Spatial Gene Expression array is used as an example.

Article Snippet: Fragmentation was carried out by incubating the samples in 32 °C for 5 min and then 65 °C for 30 min with EB and a fragmentation mix containing Fragmentation Buffer (10x Genomics, PN2000091) and Fragmentation Enzyme (10x Genomics, PN2000090).

Techniques: Gene Expression, Control, Comparison

Time comparison between the manual and automated library preparation. Times for the manual protocol are taken from the Visium Spatial Gene Expression protocol, with the reaction time subtracted from the total to get the hands-on time for the preparation of the samples. The hands-on time for the automated preparation is the preparation of the plates and the handling of the robot. The total time includes the manual handling as well as reaction time.

Journal: Heliyon

Article Title: Enabling automated and reproducible spatially resolved transcriptomics at scale

doi: 10.1016/j.heliyon.2022.e09651

Figure Lengend Snippet: Time comparison between the manual and automated library preparation. Times for the manual protocol are taken from the Visium Spatial Gene Expression protocol, with the reaction time subtracted from the total to get the hands-on time for the preparation of the samples. The hands-on time for the automated preparation is the preparation of the plates and the handling of the robot. The total time includes the manual handling as well as reaction time.

Article Snippet: Fragmentation was carried out by incubating the samples in 32 °C for 5 min and then 65 °C for 30 min with EB and a fragmentation mix containing Fragmentation Buffer (10x Genomics, PN2000091) and Fragmentation Enzyme (10x Genomics, PN2000090).

Techniques: Comparison, Gene Expression, Size Selection, Ligation